Journal: Frontiers in Microbiology
Article Title: Advances in bacteriophage-mediated strategies for combating polymicrobial biofilms
doi: 10.3389/fmicb.2023.1320345
Figure Lengend Snippet: Examples of phage treatment of polymicrobial biofilms in in vitro and in vivo studies with models.
Article Snippet: 3. , E. coli MG1655/ P. aeruginosa PAO1 , Dual-species biofilm eradication , Phage λW60 (ATCC 97537) against E. coli and phage PB-1 (ATCC 15692-B3) against P. aeruginosa , biofilm formation on silicone rubber disks placed in flasks with LB medium and inoculated with E. coli and P. aeruginosa 10 6 /mL for 2 days at 37°C with shaking, then phage treatment (MOI = 10) for 5 days with daily media refreshment , E. coli and P. aeruginosa Levels in Biofilm: Regardless of the presence of one or both phages, levels of E. coli and P. aeruginosa in the biofilm remained relatively constant Phage Resistance Development: E. coli demonstrated less resistance to its corresponding phage compared to P. aeruginosa , .
Techniques: In Vitro, In Vivo, Bacteria, Sterility, Isolation, Control, Cell Counting, Inhibition, Infection, Membrane, Incubation, Microscopy, Lysis, Recombinant, Concentration Assay, Suspension